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ps318 atg13 antibody  (Rockland Immunochemicals)
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A-C , ELISA measurement of <t>pS318-ATG13</t> signal after 16 hours of drug treatment in NCI-H2122 (A), Calu-1 (B) and NCI-H358 cell lines (C). N=3. Statistical analysis was performed using an ordinary one-way ANOVA with Tukey’s multiple comparisons test. ns= not significant, *p<0.05 ****p<0.0001. D-F, Percent confluence over time of NCI-H2122 (D), Calu-1 (E) and NCI-H358 (F) cell lines treated with indicated compounds over time. Statistical analysis was performed using an ordinary one-way ANOVA with Tukey’s multiple comparisons test. ns= not significant, *p<0.05 **p<0.01 ***p<0.001 ****p<0.0001 G-I, Immunoblotting analysis of NCI-H2122 (G), Calu-1 (H), and NCI-H358 (I) cell lines after 100nM sotorasib treatment over 24 hours.
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anti-ps318-atg13 600-401-c49  (Rockland Immunochemicals)
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A-C , ELISA measurement of <t>pS318-ATG13</t> signal after 16 hours of drug treatment in NCI-H2122 (A), Calu-1 (B) and NCI-H358 cell lines (C). N=3. Statistical analysis was performed using an ordinary one-way ANOVA with Tukey’s multiple comparisons test. ns= not significant, *p<0.05 ****p<0.0001. D-F, Percent confluence over time of NCI-H2122 (D), Calu-1 (E) and NCI-H358 (F) cell lines treated with indicated compounds over time. Statistical analysis was performed using an ordinary one-way ANOVA with Tukey’s multiple comparisons test. ns= not significant, *p<0.05 **p<0.01 ***p<0.001 ****p<0.0001 G-I, Immunoblotting analysis of NCI-H2122 (G), Calu-1 (H), and NCI-H358 (I) cell lines after 100nM sotorasib treatment over 24 hours.
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biotinylated ps318 atg13 antibody  (Rockland Immunochemicals)
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A-C , ELISA measurement of <t>pS318-ATG13</t> signal after 16 hours of drug treatment in NCI-H2122 (A), Calu-1 (B) and NCI-H358 cell lines (C). N=3. Statistical analysis was performed using an ordinary one-way ANOVA with Tukey’s multiple comparisons test. ns= not significant, *p<0.05 ****p<0.0001. D-F, Percent confluence over time of NCI-H2122 (D), Calu-1 (E) and NCI-H358 (F) cell lines treated with indicated compounds over time. Statistical analysis was performed using an ordinary one-way ANOVA with Tukey’s multiple comparisons test. ns= not significant, *p<0.05 **p<0.01 ***p<0.001 ****p<0.0001 G-I, Immunoblotting analysis of NCI-H2122 (G), Calu-1 (H), and NCI-H358 (I) cell lines after 100nM sotorasib treatment over 24 hours.
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biotinylated ps318-atg13 antibody  (Rockland Immunochemicals)
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A-C , ELISA measurement of <t>pS318-ATG13</t> signal after 16 hours of drug treatment in NCI-H2122 (A), Calu-1 (B) and NCI-H358 cell lines (C). N=3. Statistical analysis was performed using an ordinary one-way ANOVA with Tukey’s multiple comparisons test. ns= not significant, *p<0.05 ****p<0.0001. D-F, Percent confluence over time of NCI-H2122 (D), Calu-1 (E) and NCI-H358 (F) cell lines treated with indicated compounds over time. Statistical analysis was performed using an ordinary one-way ANOVA with Tukey’s multiple comparisons test. ns= not significant, *p<0.05 **p<0.01 ***p<0.001 ****p<0.0001 G-I, Immunoblotting analysis of NCI-H2122 (G), Calu-1 (H), and NCI-H358 (I) cell lines after 100nM sotorasib treatment over 24 hours.
Biotinylated Ps318 Atg13 Antibody, supplied by Rockland Immunochemicals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/biotinylated ps318-atg13 antibody/product/Rockland Immunochemicals
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anti-atg13 ps318  (Rockland Immunochemicals)
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A-C , ELISA measurement of <t>pS318-ATG13</t> signal after 16 hours of drug treatment in NCI-H2122 (A), Calu-1 (B) and NCI-H358 cell lines (C). N=3. Statistical analysis was performed using an ordinary one-way ANOVA with Tukey’s multiple comparisons test. ns= not significant, *p<0.05 ****p<0.0001. D-F, Percent confluence over time of NCI-H2122 (D), Calu-1 (E) and NCI-H358 (F) cell lines treated with indicated compounds over time. Statistical analysis was performed using an ordinary one-way ANOVA with Tukey’s multiple comparisons test. ns= not significant, *p<0.05 **p<0.01 ***p<0.001 ****p<0.0001 G-I, Immunoblotting analysis of NCI-H2122 (G), Calu-1 (H), and NCI-H358 (I) cell lines after 100nM sotorasib treatment over 24 hours.
Anti Atg13 Ps318, supplied by Rockland Immunochemicals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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atg13 ps318  (Rockland Immunochemicals)
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A-C , ELISA measurement of <t>pS318-ATG13</t> signal after 16 hours of drug treatment in NCI-H2122 (A), Calu-1 (B) and NCI-H358 cell lines (C). N=3. Statistical analysis was performed using an ordinary one-way ANOVA with Tukey’s multiple comparisons test. ns= not significant, *p<0.05 ****p<0.0001. D-F, Percent confluence over time of NCI-H2122 (D), Calu-1 (E) and NCI-H358 (F) cell lines treated with indicated compounds over time. Statistical analysis was performed using an ordinary one-way ANOVA with Tukey’s multiple comparisons test. ns= not significant, *p<0.05 **p<0.01 ***p<0.001 ****p<0.0001 G-I, Immunoblotting analysis of NCI-H2122 (G), Calu-1 (H), and NCI-H358 (I) cell lines after 100nM sotorasib treatment over 24 hours.
Atg13 Ps318, supplied by Rockland Immunochemicals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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A-C , ELISA measurement of pS318-ATG13 signal after 16 hours of drug treatment in NCI-H2122 (A), Calu-1 (B) and NCI-H358 cell lines (C). N=3. Statistical analysis was performed using an ordinary one-way ANOVA with Tukey’s multiple comparisons test. ns= not significant, *p<0.05 ****p<0.0001. D-F, Percent confluence over time of NCI-H2122 (D), Calu-1 (E) and NCI-H358 (F) cell lines treated with indicated compounds over time. Statistical analysis was performed using an ordinary one-way ANOVA with Tukey’s multiple comparisons test. ns= not significant, *p<0.05 **p<0.01 ***p<0.001 ****p<0.0001 G-I, Immunoblotting analysis of NCI-H2122 (G), Calu-1 (H), and NCI-H358 (I) cell lines after 100nM sotorasib treatment over 24 hours.

Journal: bioRxiv

Article Title: Inhibition of ULK1/2 and KRAS G12C controls tumor growth in preclinical models of lung cancer

doi: 10.1101/2024.02.06.579200

Figure Lengend Snippet: A-C , ELISA measurement of pS318-ATG13 signal after 16 hours of drug treatment in NCI-H2122 (A), Calu-1 (B) and NCI-H358 cell lines (C). N=3. Statistical analysis was performed using an ordinary one-way ANOVA with Tukey’s multiple comparisons test. ns= not significant, *p<0.05 ****p<0.0001. D-F, Percent confluence over time of NCI-H2122 (D), Calu-1 (E) and NCI-H358 (F) cell lines treated with indicated compounds over time. Statistical analysis was performed using an ordinary one-way ANOVA with Tukey’s multiple comparisons test. ns= not significant, *p<0.05 **p<0.01 ***p<0.001 ****p<0.0001 G-I, Immunoblotting analysis of NCI-H2122 (G), Calu-1 (H), and NCI-H358 (I) cell lines after 100nM sotorasib treatment over 24 hours.

Article Snippet: pS318-ATG13 antibody (Rockland Immunochemicals, 600-401-C49) was biotinylated using a Biotin Labeling Kit-NH 2 (Dojindo, LK03).

Techniques: Enzyme-linked Immunosorbent Assay, Western Blot

A, Immunoblot of NCI-H2122:ULK1 K46N cells after 24 hours of doxycycline treatment. B, Immunoblot of NCI-H2122:ULK1 K46N cells treated with 1 ug/mL doxycycline over time (hours). C, ELISA assay of pS318-ATG13 expression after 16 hours of doxycycline treatment. N=3. D, NCI-H2122:ULK1 K46N cells were engineered to express the mCherry-EGFP-LC3 reporter, and a decrease in autophagy was demonstrated after 48 hours of doxycycline treatment. N=3. All statistical significance was performed with an ordinary one-way ANOVA with Dunnett’s multiple comparisons test. *p<0.05 **p<0.01 ***p<0.001 ****p<0.0001 E, Synergy analysis of NCI-H2122:ULK1 K46N cells treated with DMSO control, sotorasib and/or doxycycline over 48 hours.

Journal: bioRxiv

Article Title: Inhibition of ULK1/2 and KRAS G12C controls tumor growth in preclinical models of lung cancer

doi: 10.1101/2024.02.06.579200

Figure Lengend Snippet: A, Immunoblot of NCI-H2122:ULK1 K46N cells after 24 hours of doxycycline treatment. B, Immunoblot of NCI-H2122:ULK1 K46N cells treated with 1 ug/mL doxycycline over time (hours). C, ELISA assay of pS318-ATG13 expression after 16 hours of doxycycline treatment. N=3. D, NCI-H2122:ULK1 K46N cells were engineered to express the mCherry-EGFP-LC3 reporter, and a decrease in autophagy was demonstrated after 48 hours of doxycycline treatment. N=3. All statistical significance was performed with an ordinary one-way ANOVA with Dunnett’s multiple comparisons test. *p<0.05 **p<0.01 ***p<0.001 ****p<0.0001 E, Synergy analysis of NCI-H2122:ULK1 K46N cells treated with DMSO control, sotorasib and/or doxycycline over 48 hours.

Article Snippet: pS318-ATG13 antibody (Rockland Immunochemicals, 600-401-C49) was biotinylated using a Biotin Labeling Kit-NH 2 (Dojindo, LK03).

Techniques: Western Blot, Enzyme-linked Immunosorbent Assay, Expressing